Histological changes in the gills, livers and muscles of Oreochromis niloticus fed with steriod, natural hormone and pawpaw seed based diets

This study was conducted to evaluate the histological changes of 17αmethyl-testosterone (MT), wet testes bull (Bos indicus), boar (Sus domesticus), bull/mud catfish (B. indicus/C. gariepinus) and pawpaw (Carica papaya) seed powder-based diet on Nile tilapia fry in an indoor experimental pond. Histological efficacies producing monosex males were determined after four months. Fifty-five assigned randomly three ponds triplicates. Five feeds composed soya-bean, fish meal, wheat bran, cassava flour formulated by adding 0.06g 17α- methyl-testosterone, 17.47g testes, 25.59g 20.48g bull/catfish 20g powder per kg feed group 1, 2, 3, 4, 5 respectively. Obtained results from morphological examination revealed a significant difference (p < 0.05) phenotypic fed with diets. The highest indistinct percentage recorded 17αmethyl-testosterone-treated while lowest diets 3 4 no differences. All groups showed histopathological differences livers, gills, muscles. However, liver histology 1 central vessels that are mildly congested abundant glycogen vacuolation somewhat fat infiltration, muscles pathological lesions including moderate severe necrosis. These alterations can be considered indication performance health culture medium indicating effects plant extract MT.